Editorial educational content. Specialist review is not claimed; consult the original references and limitations.
Definition and context
Reversed-phase liquid chromatography separates analytes by their interactions with phases of different polarity.
What to check
Changing the gradient can change retention and resolution. Compare samples under the same method.
Interpretation
Similar retention times alone do not prove identity.
Sources and verification scope
Q2(R2) Validation of Analytical Procedures ↗
FDA / ICH
Source details (original language)
- Scope
- Validation framework for analytical procedures
- Document section
- Final guidance summary
- Limitations
- Method validation does not certify this company or establish a result for a catalog lot.
- Claim verification date
- 2026-09-09
Related reading
HPLC
Liquid chromatography separates components under a defined analytical method.
Read details →Retention time
The interval associated with an analyte passing through a chromatographic system under specified conditions.
Read details →